AI funding US AI startups 2025 venture capital

The New AI Space Race - 49 US Startups Secure $100M+ in 2025

Posted on November 28, 2025 at 07:50 PM

The New AI Space Race: 49 U.S. Startups Secure $100M+ in 2025

The U.S. AI funding boom didn’t sputter in 2025 — it ignited a new era. According to TechCrunch, 49 American AI startups raised $100 million or more in 2025, matching 2024’s breakout total and reinforcing that AI is the defining startup thesis of the moment.

Forget demos and dabbling. Venture capital is now underwriting AI companies that don’t just build models — they build the full stack: infrastructure, enterprise agents, medical intelligence, robotics, and voice systems. If 2024 was AI’s coming-out party, 2025 is its national launchpad.

💰 Why This Matters

  • Investment cadence remains red hot: 49 companies crossing $100M+ funding mirrors 2024’s count, proving sustained investor conviction.
  • Repeat and mega-round funding: Several startups closed multiple rounds this year, including giant “mega-rounds” that reward long-term potential over early prototypes.
  • AI is expanding across categories — enterprise automation now stands alongside biotech, defense, and robotics as funding magnets.

The message is clear: Investors are no longer asking “Can AI work?” They’re asking “Who can ship AI at scale?”

🧠 Key Funding Themes Shaping 2026

Theme What’s Happening
🏥 Medical & Biotech AI AI diagnostic agents and drug discovery acceleration
🤖 Embodied & Robotics AI Real-world AI systems interacting in physical environments
🗣 Voice & Speech AI Multimodal AI with voice-first intelligence
🏢 Enterprise AI Agents Automating HR, legal, support, and workflows
⚙ AI Infrastructure Scalable compute, vector databases, data/model orchestration
🛡 Defense AI Autonomous systems for security and national defense

Together, these areas demonstrate an ecosystem maturing vertically while proliferating across industries.

✅ The 49 U.S. AI Startups That Raised $100M+ in 2025

  1. Absolute Labs
  2. Alation
  3. Anduril
  4. Anthropic
  5. Apogee Bio
  6. Aqua Security
  7. Atomwise
  8. Bayesian Health
  9. BigID
  10. Bright Machines
  11. Character.AI
  12. CognitiveScale
  13. Convex
  14. Crusoe
  15. Cyera
  16. DataRobot
  17. Deep Genomics
  18. Dynamic Infrastructure
  19. ElevenLabs
  20. EvolvAI
  21. Figure AI
  22. Forethought
  23. Genesis Therapeutics
  24. Glean
  25. Hebbia
  26. Hugging Face
  27. Inflection AI
  28. Injective Labs
  29. InsideTracker
  30. Insitro
  31. Jasper
  32. LatticeFlow AI
  33. Lilt
  34. MosaicML
  35. Moveworks
  36. Norm AI
  37. OpenAI
  38. Perplexity AI
  39. Pinecone
  40. Poolside
  41. Protect AI
  42. Replit
  43. Run.ai
  44. Scale AI
  45. Shield AI
  46. Speak
  47. Synthesia
  48. Unstructured
  49. Writer

🔥 What This Lineup Reveals

  • AI funding now spans every layer of the ecosystem, from developer infrastructure (Pinecone, Hugging Face, Replit) to applications (Glean, Jasper, Character.AI), to physical intelligence (Figure AI, Shield AI).
  • Foundation model competition remains open: Names like Anthropic, Inflection AI, MosaicML, and OpenAI prove AI leadership is still fiercely contested.
  • AI is stepping into the physical world: Robotics and defense startups underscore the acceleration of embodied intelligence.
  • Enterprise adoption is accelerating: Workflow agents for HR, legal, and support signal new productivity paradigms emerging.

🔮 Looking Ahead

The next 12–24 months will determine how many of these $100M-backed bets become industry platforms. Consolidation is inevitable. Utility is the new battlefield. And the winners won’t just build AI — they’ll own how the world uses it.

📖 Glossary

  • Mega-round: A much larger-than-typical funding round, signaling premium investor confidence and high valuation.
  • AI Infrastructure: Foundational compute, databases, model serving, or data orchestration layers enabling AI apps.
  • Enterprise AI Agents: AI systems capable of performing or routing complex business tasks (legal research, HR workflows, support automation, etc.).

Source: TechCrunch — U.S. AI startups funding roundup, 2025.

FEATURED TAGS
computer program javascript nvm node.js Pipenv Python 美食 AI artifical intelligence Machine learning data science digital optimiser user profile Cooking cycling green railway feature spot 景点 e-commerce work technology F1 中秋节 dog setting sun sql photograph Alexandra canal flowers bee greenway corridors programming C++ passion fruit sentosa Marina bay sands pigeon squirrel Pandan reservoir rain otter Christmas orchard road PostgreSQL fintech sunset thean hou temple in sungai lembing 海上日出 SQL optimization pieces of memory 回忆 garden festival ta-lib backtrader chatGPT generative AI stable diffusion webui draw.io streamlit LLM speech recognition AI goverance prompt engineering fastapi stock trading artificial-intelligence Tariffs AI coding AI agent FastAPI 人工智能 Tesla AI5 AI6 FSD AI Safety AI governance LLM risk management Vertical AI Insight by LLM LLM evaluation AI safety enterprise AI security AI Governance Privacy & Data Protection Compliance Microsoft Scale AI Claude Anthropic 新加坡传统早餐 咖啡 Coffee Singapore traditional coffee breakfast Quantitative Assessment Oracle OpenAI Market Analysis Dot-Com Era AI Era Rise and fall of U.S. High-Tech Companies Technology innovation Sun Microsystems Bell Lab Agentic AI McKinsey report Dot.com era AI era Speech recognition Natural language processing ChatGPT Meta Privacy Google PayPal Edge AI Enterprise AI Nvdia AI cluster COE Singapore Shadow AI AI Goverance & risk Tiny Hopping Robot Robot Materials SCIGEN RL environments Reinforcement learning Continuous learning Google play store AI strategy Model Minimalism Fine-tuning smaller models LLM inference Closed models Open models Privacy trade-off MIT Innovations Federal Reserve Rate Cut Mortgage Interest Rates Credit Card Debt Management Nvidia SOC automation Investor Sentiment Enterprise AI adoption AI Innovation AI Agents AI Infrastructure Humanoid robots AI benchmarks AI productivity Generative AI Workslop Federal Reserve AI automation Multimodal AI Google AI AI agents AI integration Market Volatility Government Shutdown Rate-cut odds AI Fine-Tuning LLMOps Frontier Models Hugging Face Multimodal Models Energy Efficiency AI coding assistants AI infrastructure Semiconductors Gold & index inclusion Multimodal Chinese open-source AI AI hardware Semiconductor supply chain Open-Source AI prompt injection LLM security AI spending AI Bubble Quantum Computing Open-source AI AI shopping Multi-agent systems AI research breakthroughs AI in finance Financial regulation Custom AI Chips Solo Founder Success Newsletter Business Models Indie Entrepreneur Growth Apple Claude AI Infrastructure AI chips robotaxi Global expansion AI security embodied AI AI tools IPO artificial intelligence venture capital multimodal AI startup funding AI chatbot AI browser space funding Alibaba quantum computing DeepSeek enterprise AI AI investing tech bubble AI investment prompt injection attacks AI red teaming agentic browsing agentic AI cybersecurity AI search AI boom AI adoption data centre model quantization AI therapy neuro-symbolic AI AI bubble tech valuations sovereign cloud Microsoft Sentinel large language models investment-grade bonds data residency